Immunologic alterations induced by a high fat diet in C57Bl/6J mice. Effect of treatment with metformin

BURGUEÑO ADRIANA; WALD MIRIAM RUTH; PROCHNIK ANDRÉS; GENARO ANA MARÍA; MARCONE PAULA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC, SAIB, SAFE, SAFIS entre 10 sociedades cientificas

Calories-rich diets in conjuction with genetical factors are key in obesity development. This disease, in addition, is a risk factor for several immunologic and metabolic alterations. Metformin - a drugused as treatment for type 2 diabetes ? is recently used to treat prediabetic obesity. In a previous study we?ve gathered evidence showing that in C57BL/6J mice, a high fat diet (HFD) increases bodyweight and desregulates glucidic metabolism. Metformin (MET) was able to reduce the body weight gain and normalize sugar metabolism. The objective of this study is to analize the effect of both HFDand MET in the immunologic response and cytokine expression. For this purpose female C57BL/6J mice were randomly divided in two groups receiving either HFD (35% fat w/w) or standard diet (SD,5% fat w/w) during 6 months. 3 months after initiated the diet, both groups were subdivided into 2 subgroups: one received MET (250 mg/kg*day) during the remaining 3 months and the other was kept as control. Immunity response was quantified by [3H]-thymidine incorporation in a mitogen-induced proliferation assay of B and T lymphocytes. Cytokines mRNA levels were determined in spleen by real-time RT-PCR, using GAPDH as housekeeper.HFD lowered proliferation of T lymphocytes (p