INFLUENCE OF CHRONIC MILD STRESS IN METABOLIC AND BEHAVIOURAL ALTERATIONS INDUCED BY A HIGH FAT DIET. INVOLVEMENT OF NEUROTROPHINS AND CYTOKINES

BURGUEÑO, ADRIANA L; GONZALES MURANO, MARIA ROSA; GENARO, ANA MARIA; PROCHNIK, ANDRES; WALD, MIRIAM RUTH

Ciudad Autonoma de Buenos Aires

Congreso; Reunion conjunta de sociedades de Biociencias; 2017

Sociedad Argentina de Investigacion Clinica

Nowadays obesity has become a worldwide disease affecting millions of people. Clinical studies have shown an association between cognitive impairment and Obesity, marking it as a risk factor for dementia development, such as Alzheimer?s disease. In a previous study we?ve gathered evidence showing that in C57BL/6J mice, a high fat diet (HFD) decreases learning and memory capacity, and desregulates sugar metabolism. Chronic Mild Stress (CMS) enhanced the metabolic desregulation while causing anxious-like behaviour.The objective of this study is to analize the role of neurotrophin and cytokines expression in behavioural alterations and their potential use as conductual disfunction markers. For this purpose one month old male C57BL/6J mice were randomly divided in two groups receiving either HFD (35% fat w/w) or standard diet (SD, 5% fat w/w) during 6 months. Two months after starting the diet, mice under HFD were randomly subdivided: One group received CMS and the other served as control. Cytokines and neurotrophins mRNA levels were determined in hippocampus and spleen by real-time RT-PCR, using GAPDH as housekeeper. In spleen, HFD+CMS increased both IL4 and IL10 mRNA levels (p