INHIBITION OF CFTR SUPPRESS PROLIFERATION OF HUMAN BREAST CANCER CELLS

CLAUZURE, MARIÁNGELES; MEDINA, V; NICOUD, MELISA; CREMASCHI, GA; TAQUEZ DELGADO, MONICA; SANTA COLOMA TA,

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Cystic fibrosis transmembrane conductance regulator (CFTR) belongs to the adenosine triphosphate (ATP)-binding cassette transporter family and is a Cl(-) and HCO3(-) conducting anion channel expressed in a wide variety of epithelial cells. Although its well-known pathophysiological role in cystic fibrosis, the exact role of CFTR in the pathogenesis of cancer remains to be elucidated. The aim of the study was to investigate the role of CFTR in proliferation and apoptosis of human breast cancer cells. Real-time (RT)-PCR was used to test the expression of CFTR. Proliferation, colony formation and apoptosis were assessed in presence or absence of specific CFTR inhibitors (CFTRinh-172 and GlyH-101) in MDA-MB-231 and MCF-7 cells. Results indicate that both CFTR inhibitors suppress colony formation in a dose dependent manner in MDA-MB-231 and MCF7 cells (P