Modulation of oxidative stress in patients with Graves' hypertyroidism. Implications on immune functionality

MACRI DELBONO R.; SABAN, MELINA; CREMASCHI, GRACIELA; KLECHA A J; DI CUGNEO, MARIANA; COSTILLA, MELISA; CURRIA, MARINA; BARREIRO ARCOS, MARÍA LAURA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica (SAIC) en conjunto con Sociedades de Biociencias

BACKGROUND: Graves´ pathology is an autoimmune disease characterized by humoral hyperreactivity against thyroid autoantigens leading to the state of hyperthyroidism. OBJECTIVE: To study the modulation of oxidative stress in peripheral blood mononuclear cells (PBMC) of patients with Graves? disease, analyzing its impact on lymphocyte functionality. METHODS: PBMC of patients with Graves´ disease (n=9) and healthy controls (n=10) were purified from peripheral blood by Ficoll-Hypaque. Reactive oxygen species (ROS) and apoptosis were evaluated by flow cytometry using DCFH-DA and Annexin-V/PI. The expression of antioxidant enzymes was analyzed by real-time PCR and western blot. Enzymatic activity was quantified by spectrophotometry. Malondialdehyde (MDA) levels were assessed by TBARs method. Proliferation was evaluated by [3H]-thymidine incorporation. RESULTS: We observed a significant increase of ROS in PBMC of patients with Graves´ hyperthyroidism (% 21.2±2.5, p