ROLE OF ANTITUMOR IMMUNITY ON CHRONIC STRESS ENHANCEMENT OF SPONTANEOUS METASTASIS OF EL4 LYMPHOMA IN MICE. EFFECT OF FLUOXETINE AND SERTRALINE TREATMENT.

MARÍA ROSA GONZALEZ MURANO; MARIA EMILIA DI ROSSO; ANA MARÍA GENARO

Ciudad Autónoma de Buenos Aires

Congreso; XLIXReunión Anual de la Sociedad Argentina de Farmacología Experimental; 2017

SAIC-SAFE-SAIB-SAI-SAA-SAB-SAFIS-SAP-SAB-SAH

(469) ROLEOF ANTITUMOR IMMUNITY ON CHRONIC STRESS ENHANCEMENT OF SPONTANEOUS METASTASIS OFEL4 LYMPHOMA IN MICE. EFFECT OF FLUOXETINE AND SERTRALINETREATMENT.María Emilia Di Rosso (1), María Rosa Gonzalez Murano (1),AnaMaría Genaro (1, 2)(1) Instituto de Investigaciones Biomédicas (BIOMED -CONICET- UCA). (2) Departamento de Farmacología, Facultad de Medicina,Universidad de Buenos Aires.Manyclinical studies have demonstrated that stress can promote the dissemination oftumor cells. Moreover, antidepressants such as fluoxetine (F) and sertraline(S) are able to modulate immunesystem and thus, tumor prognosis. We havepreviously reported that both F and S are able to prevent chronic stressenhancement of spontaneous metastasis in a murine model of lymphoma. In this context,our aim was to study the effects of chronic stress and of antidepressant treatmenton tumor invasion taking into account the role of anti-tumor immunity. For thispurpose, female C57BL/6 mice were treated with F or S and subjected (E) or not(C) to a heterotrophic chronic stress for five week and subcutaneously injectedwith EL4 cells to generate a solid tumor. Specific cytotoxic activity againsttumor cells was evaluated co-culturing spleen cells suspensions from tumorbearing mice with labeled EL4 cells. The percentage of EL4 cell lysis wassignificantly lower when incubated with splenocytes of E mice compared to Cmice (P