Inhibition of CFTR suppress proliferation of human breast cancer cells

MONICA TÁQUEZ DELGADO; TOMÁS SANTA COLOMA; MELISA NICOUD; MARIÁNGELES CLAUZURE

Capital Federal

Congreso; Reunión Conjunta de Sociedades de Biociencias - LXII Reunión Anual de la SAIC; 2017

Sociedad Argentina de Investigación Clínica

Cystic fibrosis transmembrane conductance regulator (CFTR) belongsto the adenosine triphosphate (ATP)-binding cassette transporterfamily and is a Cl(-) and HCO3(-) conducting anion channelexpressed in a wide variety of epithelial cells. Although its wellknownpathophysiological role in cystic fibrosis, the exact role ofCFTR in the pathogenesis of cancer remains to be elucidated.The aim of the study was to investigate the role of CFTR in proliferationand apoptosis of human breast cancer cells. Real-time(RT)-PCR was used to test the expression of CFTR. Proliferation,colony formation and apoptosis were assessed in presence or absenceof specific CFTR inhibitors (CFTRinh-172 and GlyH-101) inMDA-MB-231 and MCF-7 cells. Results indicate that both CFTR inhibitorssuppress colony formation in a dose dependent manner inMDA-MB-231 and MCF7 cells (P