EFFECTS OF THYROID HORMONES ON OXIDATIVE PARAMETERS IN MURINE LYMPHOCYTIC CELLS

BARREIRO ARCOS M L; COSTILLA M; CREMASCHI G A; MACRI DELBONO R; CREMASCHI G A; MACRI DELBONO R; KLECHA A J; KLECHA A J; BARREIRO ARCOS M L; COSTILLA M

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC, SAIB, SAI, SAA, SAB, SAFE, SAH, SAP

BACKGROUND: Thyroid hormones exert actions on growth, metabolism,cellular differentiation and immune functionality. OBJECTIVE:To study the effects of hyperthyroidism on oxidative parametersin murine lymphocytes. METHODS: Female BALB/c mice weretreated with T4 (12mg/l) in drinking water for 30 days (hyperthyroidanimals) or with placebo (euthyroid animals). Serum hormone levelswere quantified by radioimmunoassay. Reactive oxygen species(ROS) and apoptosis were evaluated by flow cytometry using DCFH-DA and Annexin-V/PI. The expression of antioxidant enzymesand iNOS were analyzed by real-time PCR and western blot. Enzymaticactivity was quantified by spectrophotometry. Nitrite productionwas evaluated by the Griess technique. RESULTS: We observed anincrease of ROS in lymphocytes purified from lymph node tissue(LN) and spleen (S) of hyperthyroid animals with respect to controls(29.1±2.6 % in LN and 26.2±2.1 in S, p