DEVELOPMENT AND EVALUATION OF AN IMAGING PROTOCOL TO SHOW DOXORUBICIN ADVERSE RENAL EFFECTS IN SMALL ANIMAL MODELS

ZUBILLAGA M; NICOUD M; TESAN F; SALGUEIRO JM; VANZULLI S; MEDINA VA; GIAQUINTA ROMERO D

Congreso; Reunión Conjunta de Sociedades de Biociencias - LXII Reunión Anual de la SAIC, Sociedad Argentina de Investigación Clínica; 2017

The ability of small animal imaging to show renal side effects ofa doxorubicin treatment in animal models (AM) was studied. A previouslyproven effective treatment in a breast cancer AM was assayed(ip doxorubicin, 2mg/Kg, 6 doses in 2 weeks) in two healthyAM: male Sprague Dawley rats (n=10) and Balb-c mice (n=10). Tworadiopharmaceuticals (RF) were used: 99mTc-DMSA that shows renalfunction and structure by gamma camera imaging; and 99mTc-DTPA,which shows glomerular filtration (GF) by RF retention curves.Studies were performed prior (Control) and post treatment (Dox)by intravenous injection of each RF. Serum samples were takento measure urea and creatinine levels, urine samples were taken(24h urine collected in metabolic cages) to evaluate proteinuria andkidneys were excised for histopathological evaluation. Qualitativevisual analysis of 99mTc-DMSA images showed no differences betweengroups in a species independent way. However, quantitativeanalysis showed a significant increase in kidneys, liver and jointsuptake for Dox groups in rats (P