Interplay between hyperglycemia, oxidative stress and lymphocyte proliferation on peripheral blood mononuclear cells fronm diabetic patient

FRECHTEL GUSTAVO; GEREZ ESTHER; WALD MIRIAM RUTH; LAGUARDE NATALIA; TORRACO LLANES MARTIN; SERRA HECTOR ALEJANDRO

Buenos Aires

Congreso; Reunión conjunta de Sociedades de Biociencia.; 2017

SAIC, SAFE, SAFIS, SAI SAIB y otras 10 sociedades cientificas

The association between diabetes and immunosuppression is suggested. HG is the main factor involved in diabetic complications by inducing reactive oxygen species (ROS) and oxidative stress. However, clinically there are patients with normal recovery from infections and others are susceptible. Genetic conditioning could be a factor involved. Previously we observed that lymphocytes isolated from BALB/c mice were sensitive to the deleterious effect of HG, while C57 were resistant, being oxidative stress, the main factor involved. The aim of this study was to analyze on PMBC cells from type 2 diabetic patients the interrelation between HG, oxidative stress and lymphocyte proliferation. Oxidative stress was studied by measuring glutathione content (GSH) and ROS generation. The immune function was studied by proliferation assays. The influence of HG was evaluated by preincubation of PMBC cells in a high glucose-containing medium. 32 type 2 diabetic patients were studied. Regardless of their metabolic control, there are patients who present oxidative stress (high levels of ROS) and others do not. They were divided into 4 groups according to their metabolic control (HbA1c>7,5%) and ROS production (>125%). Most individuals with high ROS, irrespective of metabolic control, age or gender, are patients whose disease is longstanding (over 9 years, p7,5% - ROS >125% increased ROS production after the incubation with high glucose medium (p