Differential effect by sex of a high fat diet in c57/bl6j mice behaviour and glucidic metabolism. Influence of chronic mild stress.

WALD MIRIAM RUTH; GONZÁLEZ MURANO MR; PROCHNIK ANDRÉS; GENARO ANA MARÍA

Mar del Plata

Congreso; Reunión Conjunta 2016 entre la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE); 2016

SAIC, SAI, SAFE

In the past decades overweight andobesity have turned into one of the most common non-contagious diseases,currently spreading worldwide.  In recentclinical studies obesity has been associated with impairment in cognitivefunction and even pointed at being a risk factor in the development ofdementias, such as Alzheimer?s disease. In the present study we analyze if ahigh fat diet can lead to behaviour alterations and wether these are associatedwith metabolic changes. Furthermore, we investigated if stress aggravates thesealterations.Male and female C57/BL6J mice werefed with a high fat diet (HFD; 35% w/w) or a standard diet (SD, 5% w/w) during6 months. Two months after starting the diet, mice under HFD were subdivided intwo groups: one received chronical mild stress (CMS) and the other was left undisturbed.By the end of the treatment we measured body weight , performed behaviour testsrelevant to anxiety, context and spacial memory and  run a glucose tolerance test.Results show that mice under HFD hada higher body weight (Male mice:N=9; p<0,05. Female mice:N=9;p<0,01  which reverted with CMS in both sex.   UnderHFD, males had glucose intolerance (p<0.05), which worsen with CMS (p<0,05)and exhibit a reduced discrimination ratio in spatial object recognition(SOR) (N=4, p<0.05) while CMS caused a worst performance in SOR and a anxious-likebehaviour  (N=9.p<0,01). We also founda negative correlation between glucose intolerance and SOR results (Correlation:-0,6257;p<0,05)and glucose intolerance and anxious-like behaviuor(Correlation:-0,4798;p<0,05). On the other hand, the treatments seem to haveno effect on females neither in their metabolism nor their behaviour. Evidenceshows that, in this model, both male and female have a body weight increasewith a HFD. However only male develop disorders associated with overweight,both metabolic and behavioural. Moreover, in malesstress exposure has a paradoxical effect preventing weight gain but increasingthe HFD deleterious effects on metabolism and behavior.