Involvement of IL-10 and TGF-β in chronically stressed mice cognitive deficit

ALEJANDRO DAVID MORONI; MARÍA LAURA PALUMBO; ANA MARÍA GENARO; MARÍA EMILIA DI ROSSO

Mar del Plata

Congreso; LXI Reunión Científica Anual de la SAIC, LXIV Reunión Anual de la SAI, XLVIII Reunión Anual de la SAFE, VII Reunión Científica Anual de la NANOMEDar y AACyTAL; 2016

SAIC, SAI, SAFE

In previous reports we found that chronic mild stress (CMS) exposure induces a decrease in learning and memory in female BALB/c mice. This cognitive deficit correlated with a decrease in CD4+CD25+FOXP3+and an increase in CD4+CD25-FOXP3+ regulatory T cells (Tregs) in CMS mice spleen. No differences in the CD4+CD25+FOXP3+ and CD4+CD25-FOXP3+ Tregs were found in lymph nodes in stressed mice. Tregs have an important role in maintaining self-tolerance through the inhibition of effector T cells. The main cytokines involved in this mechanism are IL-10 and TGF-β, both released by Treg cells. In this context, the aim of this work was to evaluate the levels of the cytokines IL-10 and TGF-β in CMS mice and its correlation with cognitive deficit. Here, we show that CMS mice presented a poor learning performance in the Y-maze (% spontaneous alternation = control: 63.52 ± 1.93; CMS: 50.46 ± 1.96; p