BIOMED   24552
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Involvement of histamine H4 receptor in the histamine-induced modulation of tumor growth and antitumor immunity in 4T1 triple negative breast cancer.
Autor/es:
DIEGO MARTINEL LAMAS; VANINA MEDINA; MELISA NICOUD; GRACIELA CREMASCHI; HELENA STERLE; JUAN CARLOS PERAZZO
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunión Anual de la SAIC, Sociedad Argentina de Investigación Clínica; 2016
Resumen:
Histamine is known to regulate cellular immune responses, affecting maturation and activity of lymphocytes and myeloid-derived suppressor cells (MDSC). However, its role in the regulation of antitumor immunity has not been well established.The aim of the present work was to investigate the influence of the lack of histamine H4 receptor (H4R) on the tumorigenic capacity, tumor cell dissemination and the antitumor immunity in response to histamine in a triple negative breast cancer (TNBC) model. For that purpose, we evaluated tumor growth parameters and the composition of splenic and lymph node immune subsets in syngeneic H4R knock-out (H4R KO) and wild-type (WT) mice, inoculated orthotopically with 4T1 murine TNBC cells. Percentages of CD4+, CD8+, CD19+, CD3+, CD11b+Gr1+ and NK1.1+ cells were quantified in spleens and lymph nodes by flow cytometry.In vitro results indicate that histamine (0.1-10 µM) reduced the cell proliferation levels of the 4T1 TNBC, evaluated by the clonogenic assay (p