HIPPOCAMPAL-RELATED BEHAVIORAL ALTERATIONS INDUCED BY PRENATAL STRESS COULDBE PARTIALLY REVERTED BY ENRICHED ENVIRONMENT.

RAMBORGER M; ZORRILLA ZUBILETE, M.A.; GENARO, A.M.; LARRECHE CALARROHANO R

Mar del Plata

Congreso; LXI Reunión Científica de la Sociedad Argentina de Investigación Clínica (SAIC),; 2016

SAIC, SAFE, SAI

During neurodevelopment, mice have an increased susceptibilityto the deleterious effects of prenatal time insults, 􀁙hich havean impact on memory, learning and anxiety􀀏related behaviors.Some of these changes are associated 􀁙ith an alteration of theH􀀲A axis response. 􀀹e aim to evaluate the effect of enrichedenvironment (EE) on hippocampal-related behavioral impairmentinduced by prenatal restraint stress.􀀲regnant female 􀀤alb/cmice 􀁙ere individually restrained 􀀔 hours/day, since the 􀀓􀀖thday of gestation until delivery. 􀀱ffspring 􀁙ere initially divided intodifferent groups􀀜 prenatal stress 􀀊􀀲S􀀋, no prenatal stress 􀀊C􀀋, inturn each group 􀁙as treated 􀁙ith EE. The offspring 􀁙ere testing􀀛􀀒 days after birth.􀀤alb/c subjected to 􀀲S sho􀁙ed impairment inhabituation memory to an 􀀱pen Field Test, and this 􀁙as revertedmeaningfully by the EE 􀀊p􀀞􀀒.􀀒􀀗􀀋. 􀀫n addition, an increase in anxietybehaviors 􀁙as observed in 􀀲S mice 􀀊p􀀞􀀒.􀀒􀀗􀀋, 􀁙hich 􀁙as revertedby EE in the elevated plus maze test. 􀀫n an 􀀱bject in place test,􀀲S performance in the discrimination tas􀁍 􀁙as altered 􀁙hencompared to control mice 􀀊p􀀞􀀒.􀀒􀀗􀀋, and EE could not revert thisdisability. 􀀯oreover, a significant increase 􀀊p􀀞􀀒.􀀒􀀗􀀋in glucocorticoidreceptors 􀀊􀀩􀀴􀀋 􀁙as found in both the hippocampus and lymphoidcells of 􀀲S mice, but 􀁙as reverted by EE, up to control levels.Corticosterone plasma levels 􀁙ere increased in acute stress miceand in 􀀲S􀀍acute stress mice, the first sho􀁙ing higher levels. EEhas proven successful in reversing this effect in the 􀀲S group. 􀀫nconclusion, these results support the idea that PS induces changesin the H􀀲A axis, 􀁙ith a subsequent altered response to stressexposure in the adult life. The behavioral alteration provo􀁍ed by􀀲S could be related to higher levels of 􀀩􀀴 in the hippocampusand lymphatic ganglia. These changes 􀁙ere partially revertedby EE. Finally, it is important to note that lymphocytes could beperipheral mar􀁍ers of H􀀲A alteration induced by 􀀲S. Funding 􀀲􀀫􀀲􀀓􀀓􀀔􀀔􀀒􀀓􀀕􀀒􀀓 􀀒􀀒􀀖􀀒􀀛 and 􀀷􀀤ACyT 􀀔􀀒􀀒􀀔􀀒􀀓􀀕􀀒􀀓􀀒􀀒􀀓􀀕􀀓