Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma.

E. CRESCENTI; VA MEDINA; LA SAMBUCO; GA CREMASCHI; AM GENARO; GP CRICCO ; G MARTÍN ; E VALLI; DJ MARTINEL LAMAS ; JC PERAZZO ; ES RIVERA; RM BERGOC

BIOLOGICAL TRACE ELEMENT RESEARCH

HUMANA PRESS INC

Lugar: Totowa, NJ; Año: 2014 vol. 157 p. 138 - 146

0163-4984

Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta venom (O-LM) on the bleomycin (BLM)-induced scleroderma mouse experimental model. C3H mice were randomly divided into four groups: control (phosphate-buffered saline (PBS)), O-LM, BLM, and BLM + O-LM. All administrations were performed subcutaneously into the back of mice. BLM was injected 5 days per week for three consecutive weeks and O-LM was administered simultaneously with BLM from the beginning of the experiments and lasted for 3 weeks after the final BLM or PBS injection (for O-LM and BLM + O-LM groups), when animals were sacrificed and histopathological, immunohistochemical, thiobarbituric acid reactive species (TBARS) evaluation, and autoantibodies detection were determined. O-LM significantly reduced BLM-induced enhanced dermal thickness (605 ± 47 vs. 956 ± 59 μm, P