IBS   24490
INSTITUTO DE BIOLOGIA SUBTROPICAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Alkaloids extract from Hippeastrum striatum (La m.) H.E. Moore: chemical analysis and antiacetylcholinesterase activity
Autor/es:
ORTIZ J.; FERESIN G.; RODRIGUEZ MATA ORLANDO; ANA I. HONFI; ZARAGOZA JD; DAVIÑA JULIO R.
Lugar:
San Miguel de Tucuman
Reunión:
Jornada; XXXVII Jornadas Argent. Botánica; 2019
Institución organizadora:
SAB
Resumen:
The Amaryllidaceae species are extensively studied for their biological activities in several pharmaceutical areas. The genus Hippeastrum has deciduous leaves, tunicated bulbs, infl orescences with two to seven fl owers of different sizes and colors and is distributed from Argentina to Mexico and the Caribbean, contributing for more than 50 of the 448 alkaloids synthesized by the family. Among them, galanthamine is widely known to be a longacting, selective, reversible, and competitive acetylcholinesterase i nhibitor alkaloid, being an important drug for the treatment of Alzheimer?s disease. As a part of the study of native Amaryllidaceae species from Argentina, the basic chloroform extract of Hippeastrum striatum (OR11, MNES), was obtained and evaluated for its inhibitory activity against acetylcholinesterase. The objective of this work was to confi rm the presence of the active galanthamine-type alkaloids galanthamine, sanguinine, and chlidanthine in the basic chloroform extract, and to evaluate its cholinesterase inhibitory activity. The thin layer chromatography analysis using reference standards of the three galanthamine-type alkaloids, showed the absence of them in the bulb extract. For the analysis of acetylcholin esterase inhibitory activity, galanthamine was used as a positive control and the results exhibited that the extract is active with an IC50 value of 29.81μg/mL. H. striatum is a promising native species for obtaining resources with anti-acetylcholinesterase action. Future assays, will provide more light about the nature of the active non-identifi ed compounds present in this species extract.