Mixoploid bulbils obtained by artificial polyploidy induction in Hippeastrum striatum (Lam.) H.E. Moore (Amaryllidaceae).

ANA HONFI; JULIO DAVIÑA; ORLANDO RODRIGUEZ

TUCUMÁN

Jornada; XXXVII JORNADAS ARGENTINAS DE BOTÁNICA; 2019

SOCIEDAD ARGENTINA DE BOTÁNICA

Colchicine is a polyploidizing agent widely used in plant breeding plans. Hippeastrum is agenus of the Amaryllidaceae native to America with great ornamental potential and as asource of phytoproducts. The records of synthetic polyploid trials in this family are scarceand of low survival. The objective of this research was to evaluate the syntheticpolyploidization of H. striatum (2n=5x=55) from Misiones - Argentina (OR18MNES). Anexperimental design was established with two bulb groups (treatment and untreated). The 30days old bulbs were immersed in an aqueous solution of 0.1% colchicine for 24 hours. Then,they were cultured in the same conditions as the controls. Six months later, the Feulgenstaining technique was used for the mitotic analysis using root tips of the treated anduntreated bulbs. The clonal progeny of these bulbs was also analyzed. The coexistence ofpentaploid (2n = 5x = 55) and decaploids cell lines (2n = 10x = 110) in the treated bulbs wasrevealed. The plants have not yet reached the reproductive stage, but the prolific vegetativeproduction of bulbils is highlighted. These bulbils have the same cytogenetic phenomenon.Mixoploidy is a chromosomal disorder characterized by the presence of two or more celllines in the same individual. This can be presented as a cytogenetic trouble that concludes inthe infeasibility of the affected individuals, but when this condition is transmitted to the nextgeneration through the agamic mode, it can become a valuable strategy that expands thepossibilities in genetic improvement plans.