Study of the genetic makeup by means of nuclear microsatellite markers of Anadenanthera colubrina (Leguminosae), a South American forest native resource

ESCALADA, MICAELA CAROLINA; GARCÍA, MARÍA VICTORIA; BARRANDEGUY, MARÍA EUGENIA; GONCALVES, ALEJANDRA LORENA

Foz do Iguaçu

Congreso; XXII International Congress of Genetics; 2018

Brazilian Genetics Society (SBG), Latin American Association of Genetics (ALAG), International Genetics Federation (IGF)

Anadenanthera colubrina (Vell.) Brenan (Leguminosae, Caesalpinioideae) known locally as curupay is a native South American species which inhabits Seasonally Dry Tropical Forests. Its current disjunct distribution represents fragments of a historical much larger area of this forest type. In Argentina, this species presents a discontinuous distribution restricted to Paranaense and Yungas phytogeographic provinces. The main aim of this study was to analyze the genetic structure of fragmented populations of Anadenanthera colubrina in Argentina. One hundred ninety two individuals from 20 natural populations were genotyping by seven specific nuclear microsatellites markers. Levels of genetic variation were estimated by standard population genetic parameters while the hierarchical genetic structure was analyzed using an Analysis of Molecular Variance (AMOVA) where populations were defined a priori based on their geographic distribution. The FST index was calculated from AMOVA. Also, population genetic structure was described using Bayesian methods. Heterozygosity was high for all loci. The mean effective number of alleles in each population was lower than the mean number of alleles, showing the presence of alleles at low frequencies in all populations. Populations from Yungas were more diverse than populations from Misiones (h=0.79-r=5.69 and h=0.82-r=5.39, respectively). Approximately 87% of the genetic variation was detected within populations, 9% among populations and 4% among provinces. FST index indicate moderate genetic structure among populations (FST=0.13, p