IBS   24490
INSTITUTO DE BIOLOGIA SUBTROPICAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
New haplotypes in populations of the exotic land snail Bradybaena similaris (Gastropoda: Bradybaenidae) from Argentina
Autor/es:
SERNIOTTI, E.N.; VOGLER, R.E.; GUZMÁN, L.B.; RUMI, A.; BELTRAMINO, A.A.; PESO, J.G.
Lugar:
Foz do Iguaçu
Reunión:
Congreso; 2018 International Congress of Genetics; 2018
Institución organizadora:
Sociedade Brasileira de Genética
Resumen:
Bradybaena similaris (Férussac, 1821), native to East Asia, is an invasive land snail species widely distributed in South America. It is catalogued as an agricultural plague, and it represents an important risk for human and animal health due to its role as intermediate host of trematodes and nematodes. At present, this species is distributed in northern Argentina with established populations in the provinces of Tucumán, Entre Ríos and Misiones. The aim of this study was to explore the genetic background of several populations from Misiones and Entre Ríos. Twenty nine individuals from nine populations were examined. Specimens were identified by the study of their shell. Total genomic DNA was isolated from fresh tissue samples by means of a CTAB protocol. Partial sequences of the mitochondrial 16S-rRNA gene were amplified by PCR using universal primers for pulmonate snails. Amplicons were purified and both DNA strands were then cycle-sequenced. The resulting sequences were trimmed to remove the primers, and the consensus sequences between forward and reverse sequencing were obtained. These sequences were compared with reference sequences in GenBank through the use of the BLASTn algorithm. Partial sequences consisted of 265-266 bp, and three haplotypes were identified within the examined populations. BLASTn search results revealed two haplotypes so far uncharacterized. The third haplotype found showed top-ranking scores and a 100% sequence identity with a sequence from Brazil. These results are expected to shed light and provide new insights on the invasion pathways of B. similaris in Argentina.