EVALUATION OF GENOTOXIC, CYTOTOXIC AND CYTOSTATIC EFFECTS OF TWO METAL ALLOYS ON HUMAN LYMPHOCYTHES

MÜLLER, S; ARES, AE; KOCIUBCZYK, AI; CAFFETTI, JD; FENOCCHIO, AS

Foz do Iguaçu

Congreso; 2018 International Congress of Genetics; 2018

Latin American Association of Genetics, Sociedade Brasileira de Genética e International Genetics Federation

Introduction: Humans are exposed to several physical and chemical agents that can affect tissues, cells or the DNA. Such is the case of metals employed in prosthesis that are used in bone traumatism treatment. Objectives: The present work proposes to evaluate the genotoxic, cytotoxic and cytostatic effects of two metal alloys used as prostheses by the cytokinesis-block micronucleus cytome assay (CBMN). Materials and Methods: Peripheral blood samples were collected from two healthy volunteers and the lymphocytes were cultured for 96 hours (37 °C). After 24 hours, cells were exposed to extracts of the metals ASTM/F745 (Stainless Steel alloy) and ASTM/F75 (Cobalt Chrome alloy) in proportion 2:1. Four repetitions were performed for each treatment. Additionally, were performed a negative control with physiological solution and a positive control with ethyl methanesulfonate (100 μl/ml). At the end of the exposure period, cells were harvested in order to perform the CBMN assay. Results and Conclusion: Only metal alloy ASTMF745 showed statistically higher genotoxicity in relation to the negative control as evaluated by micronucleus, nucleoplasmic bridges and nuclear buds frequencies. However, the cytotoxic and cytostatic effects that result from the score of apoptotic/necrotic cells and the nuclear division index respectively did not show differences from the negative control neither metal alloys (p