IBS   24490
INSTITUTO DE BIOLOGIA SUBTROPICAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Satellite DNA evolution in Scyphozoa jellyfishes (Medusozoa: Cnidaria)
Autor/es:
CASTILLO, ELIO RODRIGO D.; JONATHAN W. LAWLEY; SÓNIA CRISTINA DA SILVA ANDRADE; MARONNA, MAXIMILIANO M.; CLARISSA GARBI MOLINARI; ANDRÉ C. MORANDINI; SANTANDER, MYLENA D.; EDGAR GAMERO-MORA; SERGIO STAMPAR
Reunión:
Workshop; Evolution Meeting 2021; 2021
Resumen:
Satellite DNAs (satDNA) are fast-evolving and highly abundant repetitive sequences that representsignificant proportions of eukaryotic genomes. According to the “library” hypothesis, related species would share a common set of ancestral satellites that could evolve independently, beingdifferentially amplified and modified in each species. Scyphozoa includes 223 worldwidedistributed species of jellyfishes, and shows a striking 13-fold interspecific variation in genomessizes. Two patterns are observed within Scyphozoa: large genomes found in Coronamedusae,and smaller ones in its sister clade Discomedusae. Some studies have previously focused inScyphozoa repetitive DNA into some extent, although the evolution of satDNA has not beenaddressed yet. Here we study the satellitome of 11 species, comprising 8 Scyphozoa families from Discomedusae and Coronamedusae. Putative satellite monomers were obtained from lowcoverage samples, including novel and published NGS data. We found variability for differentsatellitome features: the number of unique satDNA families ranged from 5 to 98 per species, andmonomers length from 13bp to 5Kbp, with most monomers grouped close to the median of 180bp. Most satDNA families were species-specific, with the exception of 2 satellite families and 8 superfamilies that were shared by closely related species. Total satDNA genome proportions range from 1 to 20 percent between species, with a single satellite families occupying from 0.01 to 10% . One or two satellites were the most abundant in each species, occupying the largest proportion of the satellitome, followed by low-abundance satellites showing similar proportions. In Coronamedusae, satDNA showed lower genome percentages , and longer monomers thanDiscomedusae species. Intragenomic divergence from the consensus sequences obtained foreach family, ranged from less than 1% up to ~27% divergence, with species showing lineage-specific repeat landscapes. Thes results highlights a complex scenario of satellitome evolution,coincident with a high turnover rate of satellites in Scyphozoan species.