IBS   24490
INSTITUTO DE BIOLOGIA SUBTROPICAL
Unidad Ejecutora - UE
artículos
Título:
Phylogeny and chromosomal diversification in the Dichroplus elongatus species group (Orthoptera, Melanoplinae)
Autor/es:
CABRAL DE MELLO D.C.; MARONNA, MAXIMILIANO M.; MARTÍ, DARDO A.; CIGLIANO, MARÍA MARTA; CASTILLO ELIO R.D; MARTÍ, DARDO A.; CIGLIANO, MARÍA MARTA; CASTILLO ELIO R.D; PALACIOS-GIMENEZ O.; TAFFAREL, ALBERTO; PALACIOS-GIMENEZ O.; TAFFAREL, ALBERTO; CABRAL DE MELLO D.C.; MARONNA, MAXIMILIANO M.
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2017 p. 1 - 22
ISSN:
1932-6203
Resumen:
Inan attempt to track the chromosomal differentiationin the Dichroplus elongatus species group,we analyzed the karyotypes of four species with classical cytogenetic andmapping several multigene families through fluorescent in situ hybridization (FISH). We improved the taxon sampling of theD. elongatusspecies group adding new molecular data to infer the phylogeny of the genus andreconstruct the karyotype evolution. Our molecular analyses recovered a fullyresolved tree with no evidence for the monophyly of Dichroplus. However, we recovered several stable clades within thegenus, including the D. elongatusspecies group, under the different strategies of tree analyses (Maximum Parsimonyand Maximum Likelihood). The chromosomal data revealed minor variation in the D. elongatus species group?s karyotypescaused by chromosome rearrangements compared to the phylogenetically related D. maculipennis species group. Thekaryotypes of D. intermedius and D. exilis described herein showed the standard characteristics found inmost Dichroplini, 2n=23/24, X0♂ XX♀, Fundamental number (FN)=23/24. However, wenoticed two established pericentric inversions in D. intermedius karyotype, raising the FN to 27♂/28♀. A strongvariation in the heterochromatic blocks distribution was evidenced atinterespecific level. The multigene families? mapping revealed significantvariation, mainly in rDNA clusters. These variations are probably caused by microchromosomal changes, such as movement of transposable elements (TEs) andectopic recombination. These observations suggest a high genomic dynamism forthese repetitive DNA sequences in related species. The reconstruction of the chromosomecharacter ?variation in the FN? posits the FN=23/24 as the ancestral state, andit is hypothesized that variations due to pericentric inversions has arisenindependently three times in the evolutionary history of Dichroplus. One of these independent events occurred in the D. elongatus species group, where D. intermedius is the unique case withthe highest FN described in the tribe Dichroplini.