Análisis genómico de la cepa mosquitocida Bacillus wiedmannii biovar thuringiensis FCC41

LOPEZ RP; BERÓN, C; LAZARTE, JN

Mar del Plata

Encuentro; XIII Encuentro Biólog@s en Red; 2018

Asociación de Jóvenes Investigadores en Formación (AJIF)

Genome analysis of mosquitocidal Bacillus wiedmannii biovar thuringiensis strain FCC41LAZARTE, JN; LOPEZ, RP; BERÓN, CMBacillus cereus sensu lato also known as Bacillus cereus group is composed of an ecologically diverse bacterial group with an increasing number of related species, some of which are medically or agriculturally important. Numerous efforts have been undertaken to allow presumptive differentiation of B. cereus group species from one another. FCC41 is a Bacillus cereus group strain toxic against mosquito species like Aedes aegypti, Aedes (Ochlerotatus) albifasciatus, Culex pipiens, Culex quinquefasciatus and Culex apicinus, some of them responsible for the transmission of vector-borne diseases. Here, we report the complete genome sequence of FCC41 strain, which consists of one circular chromosome and eight circular plasmids ranging in size from 8 to 490 kb. This strain harbors six crystal protein genes, including cry24Ca, two cry4-like and two cry52-like, a parasporin gene cry41-like and multiple virulence factors. The phylogenetic analysis of the whole-genome sequence of this strain with molecular approaches such as ANI, MLST, core genome genes, rpoB and panC places this strain into Bacillus wiedmannii cluster. However, according with phenotypical characteristics such as the mosquitocidal activity due to the expression of some cry genes found in a parasporal body and encoded in plasmids of different sizes, this strain could be renamed as B. wiedmannii biovar thuringiensis strain FCC41.TRABAJO NO INÉDITO