INBIOTEC   24408
INSTITUTO DE INVESTIGACIONES EN BIODIVERSIDAD Y BIOTECNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Organellar alkaline/neutral invertases in Populus
Autor/es:
BATTAGLIA, M.E.; KOLMAN M. A.; SALERNO G. L.; BRIONES M. V.; SHARRY S. E.
Lugar:
Córdoba
Reunión:
Otro; XXXII REUNIÓN ARGENTINA DE FISIOLOGÍA VEGETAL - XVI CONGRESO LATINOAMERICANO DE FISIOLOGÍA VEGETAL; 2018
Institución organizadora:
Sociedad Argentina de Fisiología Vegetal
Resumen:
In plants, alkaline/neutral invertases (A/N-Invs) localized into the cytosol or organelles. Their key role was shown not only in growth and development but also in stress tolerance in Arabidopsis thaliana and other plants. However, the knowledge in tree species is very limited. Recent studies in Poncirus trifoliata and Populus tomentosa demonstrated that the expression of some isozymes was induced by abiotic stresses. The aim of our study is to identify stress markers in Populus, intensively cultivated as source of wood biomass and reforestation. The availability of P. trichocarpa genome allowed to retrieve sequences corresponding to the complete A/N-Inv family (Ptra-NINVs) from P. tremula. Ptra-NINs cluster in two clades (α and ß). From data obtained from subcellular localization predictors (Predotar, Target P, Wolf Psort) and phylogenetic analyses, we showed that sequences in the ß-clade grouped with characterized cytosolic A/N-Invs, while sequences in the α-clade (PtraNINV1, PtraNINV2, PtraNINV5), corresponded to isoforms targeted to mitochondria and/or chloroplasts (PtraNINV3, PtraNINV4, PtraNINV6). To characterize those organellar location, we identified the transit peptide sequences, made PtraNINV::GFP constructs and carried out transient expression in tobacco leaf cells. Later we performed preliminary experiments to identify isoforms involved in salt stress tolerance.