Characterization of an Alkaline/Neutral Invertase in development and abiotic stresses

M. BATTAGLIA; L. LECHNER; M. PEREZ GARCÍA; M.V. MARTIN; G. MARTINEZ-NOËL; G.L. SALERNO

Foz de Iguazú

Congreso; 11th International Congress of Plant Molecular Biology; 2015

International Society of Plant Molecular Biology

In plants, sucrose(Suc) and its hydrolysis products [glucose (Glc) and fructose (Fru)] have aprimary role in carbon metabolism but also as signaling transduction molecules.Invertases (Inv) catalyzed the irreversible hydrolysis to hexosEs and areclassified by their in vitro optimumpH in acid and alkaline/neutral invertases (A/N-Inv). Recent findings showed thepresence A/N-Inv not only in the cytosol but also in organelles, such aschloroplasts and mitochondria. In Arabidopsis, mitochondrial A/N-Inv gene family was represented bythree members, named as invA, invC and invH. Interestingly, analysis of knockout mutants in A/N InvA and A/N InvC genes demonstrated a new role for these proteins, showingan impaired growth phenotype with alterations in development and abiotic stressresponses.  Here, wepresent the phenotypic analysis of an Athmutant in A/N-InvH gene growing innormal and under abiotic stress conditions Relative expression of plastidic A/N-Inv genes in A/N-InvH mutant background was analyzed as well as a comparisonbetween the other mitochondrial A/N-InvC and A/N-InvA. Our results contributeto understand the role of mitochondrial A/N-Inv family proteins during developmentand stress conditions.Our results support the important role that mitochondrial A/N-Invfamily members play in plant development and under stress conditions.Supportedby CONICET, UNMdP (EXA645), PICT 1288, and FIBA.