Diferential expression of neutral/alkaline invertase isoforms in wheat tissues

LECHNER L; MARTIN MV; MARTÍNEZ NOËL G; SALERNO G

Rosario

Congreso; L Reunión Anual de Investigación en ioquímica y Biología Molecular; 2014

Alkaline/Neutral invertases (A/N-Invs) are sucrose hydrolyzing enzymes with optimum pH ranged from 6.8 to 8.0. They have been identified in plants and cyanobacteria. In plants, from being minor proteins, scarcely mentioned in comprehensive descriptions of carbon flux, they recently became leading actors in sucrose metabolism. A/N-Invs have significant roles in root morphogenesis, photosynthetic apparatus biosynthesis, and abiotic stress adaptation. In the present work we analyze by Western blot and RT-PCR the occurrence of different isoforms in leaf and root extracts from wheat seedlings after a cold treatment. The results showed that in leaves, at least, a novel A/N-Inv isoform is induced in response to low temperature. Also, the expression of wheat A/N-Inv putative genes is differentially regulated in roots and leaves in response to adverse environmental conditions. Our data support the role of A/N-Invs in relation to abiotic stresses and that they may be involved in responses to cope with them. Supported by PIP 134, UNMdP (EXA645), PICT 1288, and FIBA.