INBIOTEC   24408
INSTITUTO DE INVESTIGACIONES EN BIODIVERSIDAD Y BIOTECNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
Pseudopithomyces chartarum associated with wheat seeds in Argentina, pathogenicity and evaluation of toxigenic ability.
Autor/es:
PERELLO, ANALIA EDITH; AULICINO, MÓNICA; LABUDA, R; STENGLEIN, S.; MORENO, MV; PERELLO, ANALIA EDITH; AULICINO, MÓNICA; LABUDA, R; STENGLEIN, S.; MORENO, MV
Revista:
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2017 vol. 148 p. 491 - 496
ISSN:
0929-1873
Resumen:
Argentina is one of the top 10 world producersand exporters of wheat. In routine surveys ofwheat (Triticum aestivum L.) in Buenos Aires Province,Argentina, a new disease was observed in 2012 on seedsof wheat cv. Buck Meteoro. Symptomatic grains (blackpoints) and leaves (chlorosis and spots) were collectedduring the spring of that year. The objectives of thepresent study were to identify the causal agent, to investigateits pathogenicity in relation to nine wheat cultivarsand to identify the secondary metabolites produced byfungus. Symptomatic grains were plated on potato dextroseagar (PDA). Morphological characterization ofcolonies and sequencing of the ITS region after DNAextraction identified it as Pseudopithomyces chartarum.For pathogenicity tests, two different isolates, P221 andP224, were inoculated on seedlings of nine wheatcultivars, which showed different disease symptoms,% of grain germination (GG), % of grain discoloration(GD) and % of weak seedlings (WS), suggesting differentlevels of response against Pseudopithomyceschartarum. Particularly B. Meteoro, Buck Guapo andKlein Proteo cultivars demonstrated major infectiontolerance for GD and WS. In contrast, Sy 100 and KleinPantera were most affected showing weakness, chlorosis,or reduced length of coleoptile, and 50% of necroticsymptoms. Pseudopithomyces chartarum isolates producedsecondary metabolites including alternariol,alternariol mono-methyl ether, altertoxin I andaltertoxine II. The fungus is a new pathogen of wheatin Argentina that can cause diseases on different cultivarsas well as produce mycotoxins.