INBIOTEC   24408
INSTITUTO DE INVESTIGACIONES EN BIODIVERSIDAD Y BIOTECNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories
Autor/es:
JUAN CF ORTIZ MÁRQUEZ; MAURO DO NASCIMENTO; LEONARDO CURATTI
Revista:
METABOLIC ENGINEERING
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 23 p. 154 - 164
ISSN:
1096-7176
Resumen:
The biologicalnitrogen fixation carriedoutbysomeBacteriaandArchaeaisoneofthemostattractive alternativestosyntheticnitrogenfertilizers.Inthisstudywecomparedtheeffectofcontrollingthe maximum activationstateofthe Azotobacter vinelandii glutamine synthasebyapointmutationatthe activesite(D49Smutation)andimpairingtheammonium-dependenthomeostaticcontrolofnitrogen- fixation genesexpressionbythe ΔnifL mutation onammoniumreleasebythecells.Strainsbearing the singleD49Smutationweremoreefficient ammoniumproducersundercarbon/energylimiting conditions andsustainedmicroalgaegrowthattheexpenseofatmosphericN2 in syntheticmicroalgae? bacteria consortia.Ammoniumdeliverybythedifferentstrainshadimplicationsforthemicroalga┬┤s cell-size distribution.Itwasuncoveredanextensivecrossregulationbetweennitrogen fixation and assimilation thatextendscurrentknowledgeonthiskeymetabolicpathwayandmightrepresent valuablehintsforfurtherimprovementsofversatileN2-fixing microbial-cellfactories.