IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Toxic effects of endosulfan based insecticides on mammalian sperm: evaluation of in vitro chromatin decondensation and dna fragmentation
Autor/es:
SANCHEZ, M.; ALVAREZ SEDÓ, C.; ROMANATO, M.; LO NOSTRO, F.; CALVO, L.; CALVO, J.C.; FONTANA, V.
Lugar:
Porto
Reunión:
Congreso; 51st Congress of the European Societies of Toxicology, Eurotox; 2015
Institución organizadora:
Eurotox
Resumen:
Endosulfan is an organochlorine insecticide, which is extensively used in several countries to protect crops from pests. Even though a global ban on the manufacture and use of endosulfan was negotiated under the Stockholm Convention because of its threats to human health and the environment, it is still used in some countries. As several studies support that endosulfan can affect human development, the aim of this study was to analyze whether endosulfan could alter mouse sperm chromatin decondensation, which occurs in the ooplasm following oocyte penetration by the sperrmatozoon. Cauda epididymal spermatozoa were obtained from adult male mice (60-70 days old) and incubated in the presence of each of two commercial formulations (CF) of endosulfan (Master® and Zebra Ciagro®), the active ingredient (AI) alone or DMSO (control). Incubation with endosulfan significantly decreased (p< 0.0001) sperm % motility with respect to control sperm. Sperm in vitro decondensation was evaluated following exposure to endosulfan and compared to decondensation achieved in the presence of glutathione and heparin (control). Spermatozoa incubated with AI (36 ± 3% control versus 60.5 ± 5% treated, n=7, p