IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
FASTING HEPATIC RESPONSE IN MALES AND FEMALES OF Cichlasoma dimerus
Autor/es:
KARP, PAOLA; PEREZ SIRKIN, DANIELA; DELGADÍN TH; DI YORIO, PAULA; VISSIO, PAULA G.
Lugar:
Chascomús
Reunión:
Jornada; XVI JORNADAS DE LA SOCIEDAD ARGENTINA DE BIOLOGIA; 2014
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
The liver is a key organ to study the health and nutritional status of an animal. However, the hepatic histological changes that occur in a fasting situation are not well characterized in fish. The aim of this study was to analyze the effect of fasting on liver histology in C. dimerus. Fish pairs at the same reproductive stage (two days after spawning) were separately maintained for three weeks in small aquaria. During this period, the animals were daily fed with commercial pellets at 1.5% of their body weight or completely unfed. At the end of the three weeks period, morphological parameters, hepatosomatic indeces (HSI) and liver histology were studied. Fasted males and females C. dimerus presented lower HSI (p=0.0003) and hepatocyte area (p=0.0004) compared to fed ones. In adittion, differences were observed in hepatocytes areas between males and females of the same treatment (p=0.04). On the other hand, in unfed fish, an increase in melanomacrophages centers, an indicator of stress was observed. Finally, an increase in apoptotic cells visualized with TUNEL and active caspase-3 immunohistochemistry techniques was observed in unfed fish. In conclusion these results show that fasting induce quantifiable changes in the liver that include, among others, cell apoptosis.