OOCYTE-EMBRYONIC ANOMALIES INDUCED BY PERICONCEPTIONAL ALCOHOL CONSUMPTION. EFFECTS OF SUPEROVULATION IN A PRECLINIC EXPERIMENTAL MURINE MODEL

GUALDONI GISELA ; CEBRAL ELISA; JACOBO PATRICIA; BARRIL CAMILA

Simposio; First International Symposium on Reproductive Health: Overcoming barriers for research in reproduction? (ISRH); 2021

The effects of low-moderate periconceptional alcohol consumption on oocyte-embryonic quality are poor understood. With the objectives of analyzing the impact of perigestational alcohol intake on organogenic embryo development and potential gametic damage as an etiological factor for embryonic anomalies, we designed a murine experimental model with spontaneous ovulation or superovulation of ethanol administration from before fertilization to early gestation. Mouse adult females (60 days) (CrlFcen:CF1, Bioterio Central de FCEN) were administered for 15 days with ethanol 10% in drinking water (treated females, TF) or water (control females, CF) and were superovulated (5 IU/female of eCG/hCG, CFs and TFs) or not (spontaneous ovulation, CFe and TFe). After mating (day 1 of gestation, D1), ethanol administration continued until D10 in TF (D10-CFs/TFs and D10-CFe/TFs). At D10, TFs had reduced number of implantation sites vs CFs (p