IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
artículos
Título:
Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity
Autor/es:
HONJI, R. M.; CANEPPELE, D.; PANDOLFI, M.; LO NOSTRO, F. L.; MOREIRA, R. G.
Revista:
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Lugar: New York; Año: 2015 vol. 298 p. 1644 - 1658
ISSN:
1932-8486
Resumen:
The aim of this study was to identify and characterize pituitary cells ofSteindachneridion parahybae females in captivity, highlighting the possiblerelationship with reproductive disorders at this level, since this species showsoocyte final maturation, ovulation and spawning dysfunction in captivity. Thelocalization and distribution of growth hormone (GH), prolactin (PRL), somatolactin(SL), b-luteinizing hormone (b-LH), and b-follicle stimulating hormone(b-FSH) immunoreactive (-ir) cells in the adenohypophysis was studiedby immunohistochemical and Western blot methods. In addition, cellularmorphometric analyses and semi-quantification of ir-cells optical density(OD) during the annual reproductive cycle and after artificial induced spawning(AIS) were performed. Results showed that the distribution and generallocalization of pituitary cell types were similar to that of other teleost species.However, the morphometrical study of adenohypophysial cells showed differencesalong the reproductive cycle and following AIS. In general, females atthe vitellogenic stage presented greater OD values for GH, PRL and SL thanat other maturation stages (previtellogenic and regression stages), probably indicating an increased cellular activity during this stage. Conversely, b-LHOD did not vary during the annual reproductive cycle. After AIS, b-LH, SLand GH ir-cells showed an increase in OD values suggesting a possibleinvolvement on oocyte final maturation, ovulation and spawning or a feedbackcontrol on the brain-pituitary-gonads axis. Reproductive dysfunction inS. parahybae females in captivity may be due to alteration of the synthesispathways of b-LH. In addition, GH family of hormones could modulate associatedmechanisms that influence the reproductive status in this species.