Carbon and Nitrogen Sources Influence Tricalcium Phosphate Solubilization and Extracellular Phosphatase Activity by Talaromyces flavus

STEFANONI RUBIO P. J.; GODOY MANUEL SANTIAGO; DELLA MONICA I. F. ; PETTINARI, M. J. ; GODEAS A. M. ; SCERVINO J. M.

CURRENT MICROBIOLOGY

SPRINGER

Lugar: Berlin; Año: 2015 vol. 71 p. 1 - 12

0343-8651

The aim of this work was to study phosphate (P) solubilization (and the processes involved in this event) by Talaromyces flavus (BAFC 3125) as a function of carbon and/or nitrogen sources. P solubilization was evaluatedin NBRIP media supplemented with different carbon (glucose, sorbitol, sucrose, and fructose) and nitrogen (L-asparagine, urea, ammonium sulfate (AS), and ammonium nitrate (AN) combinations. The highest P solubilization was related to the highest organic acid production (especially gluconic acid) and pH drop for those treatments where glucose was present. Also P solubilization was higher when an inorganic nitrogen source was supplemented to the media when compared to an organic one. Although not being present an organic P source, phosphataseactivity was observed. This shows that P mineralization and P solubilization can occur simultaneously, and that P mineralization is not induced by the enzymesubstrate. The combination that showed highest P solubilization was for AN-glucose. The highest acid phosphatase activity was for AS-fructose, while for alkaline phosphatase were for AS-fructose and AN-fructose. Acid phosphatase activity was higher than alkaline. P solubilization and phosphatase activity (acid and alkaline) were influenced by the different carbon?nitrogen combinations. A better understanding of phosphate-solubilizing fungi could bring a better use of soil P.