IBBEA 24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
artículos
Título:
Intraspecific diversity of the arbuscular mycorrhizal fungus Glomus intraradices in extraradical mycelium development and sporulation dynamics under monoxenic cultures
Autor/es:
VANESA SILVANI; LAURA FERNANDEZ BIDONDO; M. JOSEFINA BOMPADRE; ROXANA COLOMBO; MARIANA PERGOLA,; AGUSTIN BOMPADRE; SEBASTIAN FRACCHIA; ALICIA GODEAS
Revista:
MYCOLOGIA.
Editorial:
ALLEN PRESS INC
Referencias:
Lugar: Lawrence; Año: 2014 vol. 106 p. 963 - 975
ISSN:
0027-5514
Resumen:
The intraspecific variability in the dynamic growth of extraradical mycelium (ERM) and spores of the arbuscular mycorrhizal (AM) fungus Glomus intraradices was evaluated after following the developmental kinetics of both extraradical structures under in vitro conditions.
Fourteen geographically different isolates of this AM fungal species were monoxenically grown in association with Ri T-DNA transformed carrot roots. The temporal dynamics of extraradical structures was evaluated by fitting each growth curve with the modified Gompertz model. Fungal growth parameters (lag time, maximum rate, and maximum ERM/spore production) were estimated for each isolate, and statistically compared among isolates.
The application of the Gompertz function successfully modeled in vitro growth curves of ERM and spores. A sigmoid development pattern with three recognizable phases (lag, exponential and stationary phases) was observed in all isolates. Intraspecific variability in the development kinetics of extraradical structures was detected and growth parameters were statistically different among G. intraradices isolates. The sporulation dynamics was delayed in time with respect to the growth dynamics of external mycelia in most of the G. intraradices isolates, and only two isolates developed both extraradical structures synchronously.
Therefore, different AM fungal growth patterns were found in G. intraradices isolates from diverse natural environments. This phenotypic variability suggests genetic differences within G. intraradices isolates from different populations and could be related to the different life-history strategies of AM fungi.
