INTERACTION OF RstBA AND RcscDB REGULATORY

LOPEZ, FE; ANDRÉS GRAMAJO; CABEZA ML; GARCÍA VÉSCOVI E; MORERO RD,; DELGADO, MONICA ALEJANDRA

Puerto Madryn, Argentina

Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en; 2010

Sociedad Argentina de Investigación en

INTERACTION OF RstBA AND RcscDB REGULATORY SYSTEMS FOR CONTROL OF THE Salmonella typhimurium López Fe1, Gramajo A1, Cabeza ML2, García Véscovi E2, Morero RD1, Delgado MA1 1Dpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com1, Gramajo A1, Cabeza ML2, García Véscovi E2, Morero RD1, Delgado MA1 1Dpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com1, Delgado MA1 1Dpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.comDpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.comSalmonella typhimurium López Fe1, Gramajo A1, Cabeza ML2, García Véscovi E2, Morero RD1, Delgado MA1 1Dpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com1, Gramajo A1, Cabeza ML2, García Véscovi E2, Morero RD1, Delgado MA1 1Dpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com1, Delgado MA1 1Dpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.comDpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com López Fe1, Gramajo A1, Cabeza ML2, García Véscovi E2, Morero RD1, Delgado MA1 1Dpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com1, Gramajo A1, Cabeza ML2, García Véscovi E2, Morero RD1, Delgado MA1 1Dpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com1, Delgado MA1 1Dpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.comDpto. Bioquimica. de la Nutrición, INSIBIO - CONICET - UNT, Tucumán. 2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com2IBR-CONICET, U.N.R., Rosario. E-mail: monaledel@hotmail.com The two-component regulatory systems are one of the most important mechanisms by which bacteria have managed to survive environmental changes, by modulating the gene expression. The aim of this study was to determine if there is an interaction between the RstBA and RcsCDB regulatory system to control the expression of different genes. This study was based on the observation that RstA (over-production only) negatively regulates csgD, whose product modulates those genes required for cell-cell interactions and their environment, like bapA. On the other hand, the csgD gene is positively regulated by RpoS, which in turn is repressed by RstA and induced by RcsB. This led us to think that RstBA and RcsCDB systems may be interacting in order to control genes, especially those required by Salmonella to establish systemic infection. In this work we investigated the effect of overexpression of RcsB on gene fusions controlled by RstA, such as asr, narZ and spvA. Our results indicate that RcsB and RstA competitively inhibit the narZ andcsgD, whose product modulates those genes required for cell-cell interactions and their environment, like bapA. On the other hand, the csgD gene is positively regulated by RpoS, which in turn is repressed by RstA and induced by RcsB. This led us to think that RstBA and RcsCDB systems may be interacting in order to control genes, especially those required by Salmonella to establish systemic infection. In this work we investigated the effect of overexpression of RcsB on gene fusions controlled by RstA, such as asr, narZ and spvA. Our results indicate that RcsB and RstA competitively inhibit the narZ andbapA. On the other hand, the csgD gene is positively regulated by RpoS, which in turn is repressed by RstA and induced by RcsB. This led us to think that RstBA and RcsCDB systems may be interacting in order to control genes, especially those required by Salmonella to establish systemic infection. In this work we investigated the effect of overexpression of RcsB on gene fusions controlled by RstA, such as asr, narZ and spvA. Our results indicate that RcsB and RstA competitively inhibit the narZ andSalmonella to establish systemic infection. In this work we investigated the effect of overexpression of RcsB on gene fusions controlled by RstA, such as asr, narZ and spvA. Our results indicate that RcsB and RstA competitively inhibit the narZ andasr, narZ and spvA. Our results indicate that RcsB and RstA competitively inhibit the narZ andnarZ and spvA genes transcription. Furthermore, RcsB represses asrgenes transcription. Furthermore, RcsB represses asr expression by inhibiting the rstA transcription.rstA transcription.