INVESTIGADORES
DELGADO Monica Alejandra
congresos y reuniones científicas
Título:
Increase in the virulence fitness of Shigella flexneri: the NOA population?s prevalent pathogen
Autor/es:
TORRES LAMBERTI, MF; BALLESTEROS, MARÍA F.; BONANO, MARCELA; PESCARETTI MM,; DELGADO MA
Lugar:
ENTRE RIOS- PARANA
Reunión:
Congreso; 54th Reunión Anual de la Sociedad Agentina de Investigación Bioquímica y Biología Molecular (SAIB); 2018
Institución organizadora:
SAIB
Resumen:
The Ministry of Health of the Nation, informed that in Argentina almost of 1,200,000 children suffer diarrhea disease per year. These cases aredirectly related to the incidence of enteropathogens as Shigella, the main etiological agent of foodborne diseases (FBD).It was reported that 30%of these cases are register in the northwest of the country (NOA). In previous studies, we have determined Shigella flexneri 2 as the prevalentpathogen responsible of infantile diarrhea in the NOA region. Here, we perform in vitro studies of two clinical isolates (CI) from our bank ofstrains, CI133 and CI172, isolated in 2013 and 2017 respectively. On the bases that Shigella causes piroptosis in macrophages to reach thebasolateral cells of the intestinal epithelium, we determined the cytotoxicity generated by these strains in a Caco-2 and RAW 264.7 cell linesinfection model. For this purpose, we determined the levels of lactate dehydrogenase (LDH) after different infection times with the clinicalisolates CI133 and CI172, using Shigella flexneri isolated during 2000, as reference. On the other hand, we performed in vitro infection assayusing the same cell lines to determine the virulence capacity of these isolates. We observed that the CI133 and CI172 strains showed increasedcytotoxicity levels related to those observed in the reference strain. In concordance, both isolates displayed higher replicative power withinCaco-2 cells than the control, while their elimination from phagocytic cells occurred faster. Taken together, these results allowed us to suggestthat the virulence fitness of the FBD?s prevalent pathogens is improved over time, in the NOA population.