INVESTIGADORES
DELGADO Monica Alejandra
congresos y reuniones científicas
Título:
Development and optimization of an attenuated vaccine designed to control Salmonella infection.
Autor/es:
MARIELA A. TORRES; M. F. TORREZ LAMBERTI; JUAN V. FARIZANO; MARIA DE LAS MERCEDES PESCARETTI; C. J MINHAK ; DELGADO, MONICA ALEJANDRA
Reunión:
Congreso; Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología molecular; 2013
Institución organizadora:
Sociedad Argentina de Investigaciones en Bioquímica y Biología molecular
Resumen:
Foodborne diseases (FBD) are highly related to the low income areas and represent one of the main problems worldwide. The most common causative agent of FBD is Salmonella. The process of Salmonella infection to non-phagocytic cells (epithelial cells) involves the adhesion, invasion, maturation of Salmonella-containing vacuoles and replication steps. The RcsCDB regulatory system is deeply involved in controlling the expression of genes required to establish these stages. In our laboratory, we have obtained the rcsC11 attenuated mutant, affecting the RcsC sensor, which causes constitutive activation of the system. In this study we evaluated the ability of both the wild-type strain and the rcsC11 mutant of S. Typhimurium to infect epithelial cells (non-phagocytic) and macrophage cells (phagocytes). We determined that the rcsC11 attenuated mutant was capable of producing a strong immunogenic response. Afterward, the mutant was gradually removed because it was unable to replicate in macrophages, in contrast to other well described Salmonella attenuated mutants. Furthermore, we constructed the pMAT13 plasmid vector that was able to replicate in S. Typhimurium. The rcsC11 mutant transformed whit pMAT13 were able to express and secrete heterologous proteins. This finding indicates that the rcsC11 mutant is a good candidate for the development of new vector vaccines.