INVESTIGADORES
DELGADO Monica Alejandra
congresos y reuniones científicas
Título:
THE SBMA LOCUS IS INVOLVED IN VIRULENCEASSOSIATED PHENOTYPES
Autor/es:
CORBALAN, NS; POMARES MF; LAMI, MJ; ADLER, C; DE CRISTOBAL, RE; DELGADO, M. A.;; VINCENT, PA
Lugar:
Potrero de Funes, San Luis
Reunión:
Congreso; . XLVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología molecular; 2011
Resumen:
SbmA protein is involved in the transport of MccB17, MccJ25, bleomycin and proline rich peptides into the Escherichia coli cytoplasm. In Brucella abortus, Sinorhizobium meliloti and Mycobacterium tuberculosis the SbmA homologue, BacA, is essential for the chronic infection of these pathogens. We evaluated SbmA role in the Salmonella Typhimurium ability to infect and replicate within macrophages. The sbmA mutant strain showed increased intracellular replication compared to parental strain. Further assays demonstrated that this phenomenon was caused by increased resistance to the acidic pH present in the macrophage environment. Biofilm formation, aggregation and swarming are important bacterial traits that affect pathogen chances of survival in competitive environments and the ability to infect hosts. Assays demonstrated that sbmA absence significantly enhanced biofilm, aggregation and swarming phenotypes. Additionally, mutation in yaiW, gene transcriptionally related to sbmA, affected biofilm and swarming in the same way as sbmA mutation did. However, the yaiW mutant showed a reduction in the aggregation phenotype. A gene profile expression assay of sbmA and yaiW mutans showed that the transcription levels of genes implicated in swarming and motility were enhanced in those mutants. These results suggest that sbmA is involved in important physiological roles related to virulence.