The tolC locus affects the expression of sbmA through σE activity increase

NATALIA S. CORBALÁN; CONRADO ADLER; RICARDO E. DE CRISTÓBAL; MARÍA FERNANDA POMARES; MÓNICA A. DELGADO; PAULA A. VINCENT

FEMS MICROBIOLOGY LETTERS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Holanda; Año: 2010 vol. 311 p. 185 - 192

0378-1097

SbmA protein is involved in the transport of MccB17, MccJ25, bleomycin and proline rich peptides into the Escherichia coli cytoplasm. sbmA gene homologues were found in a variety of bacteria. However, the physiological role of this protein is, until now, unknown. Previously, we found that a combination of sbmA and tolC mutations in Tn10?carrying E. coli K-12 strains results in hypersusceptibility to tetracycline. In this work, we studied sbmA expression in a tolC mutant background and observed an increased expression throughout growth. We ruled out the global transcriptional regulator RpoS and the sRNA micF as intermediates in this regulation. tolC mutation induced the expression of other well characterized strong σE -dependent promoters in E. coli. We observed that the increase in σE activity led to greater sbmA expression and, conversely, eliminating σE prevented expression of sbmA. We also observed that the sbmA upregulation in a tolC mutant context was abolished in an rpoE-null strain. These results suggest a σE-dependent positive regulation on sbmA by tolC mutation. We hypothesize that this mechanism might be part of a compensatory cell envelope stress response.