INVESTIGADORES
CHABAY Paola Andrea
artículos
Título:
Epstein Barr virus associated pediatric nasopharyngeal carcinoma. Its correlation with p53 and bcl-2 expression
Autor/es:
PRECIADO, M. V.; PAOLA ANDREA CHABAY; DE MATTEO, E; ZUBIZARRETA, P.; GALLO, G.; REY, G.; GRINSTEIN, S.
Revista:
Medical and Pediatric Oncology
Editorial:
Wiley Interscience
Referencias:
Año: 2002 vol. 38 p. 345 - 348
ISSN:
0098-1532
Resumen:
Background Pediatric nasopharyngeal carcinoma (NPC) is rare in most countries The Epstein Barr virus (EBV) association with the oncogenesis of NPC is well established. Apoptosis-related proteins, p53 and bcl-2, have been previously related to adult NPC pathogenesis.  Procedure: From 1988 to 1998, 16 patients were treated with NPC at Hospital de Niños R.Gutiérrez and Hospital de Pediatría J P Garrahan. Their median age was 12 years (range 8-20), 2 female and 14 male. We studied the presence of p53, bcl-2 and latent membrane protein-1 (LMP-1) of EBV expression by immunohistochemistry and Epstein Barr encoded RNAs (EBERs) in situ hybridization in tissue sections from formalin-fixed, paraffin-embedded NPC biopsies. Results: EBV presence and LMP-1 expression in tumor epithelial cells were detected in all the biopsies studied. P53 was expressed in 13/16 NPCs, but the frequency of positive malignant cells differed from case to case, ranging from less than 25 to 100% with heterogeneous staining intensity. Bcl-2 positive staining in tumor epithelial cells was detected in 2/16; whereas 10/16 cases showed bcl-2 positivity in infiltrating lymphocytes. Conclusions: Although our series is small, we conclude that the high percentage of p53 detection in our cases might support a role for p53 in the pathogenesis pediatric EBV associated NPC, meanwhile the association with bcl-2 is very low suggesting that it is not a crucial oncoprotein in the development of pediatric NPC.