Low frequency of Epstein Barr virus association and high frequency of p53 overexpression in an Argentinean pediatric T-cell lymphoma series.

PAOLA ANDREA CHABAY; DE MATTEO E; LORENZETTI, M.; VIJNOVICH, A.; VALVA, P.; PRECIADO, M. V.

PEDIATRIC AND DEVELOPMENTAL PATHOLOGY

Springer

Año: 2009 vol. 12 p. 28 - 34

1093-5266

Background: T-cell Non-Hodgkin´s lymphomas represent 10-15% of all diagnosed lymphomas in Western countries. Various geographic frequencies of T-cell NHL have been documented, in part reflecting increased exposure to pathogenic factors such as Epstein Barr virus (EBV). Aims: to assess EBV and p53 expression in Argentine pediatric T-cell lymphoma, and to correlate them with patients´ survival. Procedure: EBERs in situ hybridization and LMP1 and p53 immunohistochemical staining were performed on formalin-fixed paraffin-embedded lymph node biopsies from 25 pediatric T-lymphoma patients. In 17/25 samples good quality DNA was obtained and EBERs PCR was assessed to confirm in situ hybridization and immunohistochemical results. Results: EBV expression was found in 8.0 % of cases. P53 positive staining was distributed in 92% of pediatric cases. Kaplan Meier survival analysis showed that neither EBV nor p53 expression was statistically significant associated with EFS. Conclusions: Our data showed a low frequency of EBV association with pediatric T-cell. It seems that p53 plays an important role in proliferation in our studied population, since it is overexpressed in 92% of T-cell lymphoma cases.