Effect of High Pressure Treatment on Structural Properties of Low Density Lipoprotein of Hen Egg Yolk

MARIA CECILIA PUPPO; NICOLAS CHAPLEAU; FRANCISCO SPERONI; MARIE DE LAMBALLERIE; MARIA CRISTINA AÑÓN; MARC ANTON

Montpellier Francia

Congreso; ICEF 9; 2004

Changes in LDL structure of acidic (LDL3) and alkaline (LDL8) dispersions (1% w/v) due to HP treatment (200-600 MPa) was studied. Protein structure changes and aggregation phenomenon were analyzed by protein solubility, surface hydrophobicity, free sulfhydryls, circular dichroism, size exclusion chromatography, and quasi-elastic light scattering and native electrophoresis. Secondary structure changes followed by aggregation were observed for LDL8 at 600 MPa. This behavior was accompanied with a decrease in H0 and an increase in SHF. No effect on LDL3 structure with HP was observed.