Development and preliminary validation of an antibody filtration-assisted single-dilution chemiluminometric immunoassay for potency testing of Piscirickettsia salmonis vaccines.

WILDA, MAXIMILIANO; LAVORIA, MARÍA DE LOS ÁNGELES; GIRÁLDEZ, ADRIÁN; FRANCO MAHECHA, OLGA; MANSILLA, FLORENCIA; ERGUIS, MATÍAS; IGLESIAS, MARCELA; ALEJANDRA CAPOZZO

BIOLOGICALS

ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2012 vol. 40 p. 415 - 420

1045-1056

Challenge with live pathogens could be substit uted by serology for ma ny veterinar y diseases, however little progress has been made in the development of alternative batch-vaccine potency tests for fish. This study reports the development and preliminary validation of a single-dilution filtration-assisted chemiluminometric im munoassay (SD FAL-ELISA) applied to measure anti Piscirickettsia salmonis IgM in individual or pooled serum and mucus samples. The assay was set up to test a single-dilution of the sample. Serum SD FAL-ELISA yielded a sensitivity of 90% and a specificity of 96 %. SD FAL-ELISA was applied to evaluate pooled and individual samples from P. salmonis challenge assessments. Relative-light units values (RLU) obtained by SD FAL-ELISA were proportional to antibody levels in serum. RLU values obtained from pooled and individual serum samples increased wi th the observed relative percent survival (RPS) values, indicating a correlation between protection and specific IgM levels. Results obtained for speci fic IgM in mucus samples were not related to the RPS, but discriminated the vaccine that yielded high RPS (86. 4%) from the others (40.9 an d 54.5%). This is the fi rst report on the development of an indirect high-throughput serological assessment for P. salmon is vaccine potency testing using both pooled or individual serum and cutaneous mucus samples.