INVESTIGADORES
GIACOMODONATO Monica Nancy
artículos
Título:
Dam methylation regulates the expression of SPI-5-encoded sopB gene in Salmonella enterica serovar Typhimurium
Autor/es:
MONICA N. GIACOMODONATO; MARIANGELES NOTO LLANA; MARIA DEL ROSARIO AYA CASTAÑEDA; FERNANDA BUZZOLA; MARINA GALLO CALDERON; MARIA C. CERQUETTI
Revista:
MICROBES AND INFECTION
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 16 p. 615 - 622
ISSN:
1286-4579
Resumen:
DNA adenine methylation is an essential factor in Salmonella virulence. Here, we investigate the involvement of DNA adenine methylase(Dam) in the expression and translocation of a SPI-5-encoded effector of S. Typhimurium. SopB expression and secretion were determined usingSopBeFLAG-tagged wild type and dam strains of S. Typhimurium. Western blot and quantitative reverse transcriptase PCR analysis showed thatthe dam mutant expresses lower levels of SopB protein and sopB mRNA than the wild type strain under SPI-1 and SPI-2 inducing conditionsin vitro. SopB secretion was also considerably impaired in the absence of dam. In agreement with in vitro experiments, SopB synthesis in dammutants recovered from infected epithelial cells and from murine mesenteric lymph nodes was reduced by 40% respect to the wild type strain( p < 0.05). SopB translocation was neither detected in the cytosol of epithelial cells nor in the cytosol of cells isolated from mesenteric lymphnodes infected with the dam mutant. Taken together, our results demonstrate that, in S. Typhimurium, Dam methylation modulates the expressionand translocation of SPI-5-encoded SopB effector