Análisis comparativo de poblaciones de retrotransposones LTR que habitan los genomas de Solanum pennelli y Solanum Lycopersicum

YAÑEZ ANAHÍ; NATALIA PILAR ANDINO; PAZ, ROSALÍA CRISTINA

San Juan

Congreso; II Jornadas Argentinas de Jóvenes Botánicos; 2016

UNSJ

Solanumpennelli is a wild tomato species endemic to Andean regions in South America. Meanwhile,Solanum lycopersicum is a domesticatedspecies of high economic value for its agronomical importance. Both species arehighly divergent within the Solanaceae´s family, but are still related enoughto produce viable progeny. The haploid genome size of S. pennellii has been estimated to be ~1200 Mb, and in S. lycopersicum ~950 Mb, where differences in genome size are mainly attributed to theaccumulation of LTR retroelements. We performed a genome-wide LTR retroelement annotationbased on structural, functional and time-insertion selection for theidentification of potentially active retroelements. We identified numericaldifferences and Copia:Gypsy (C:G) ratios between the genomes, containing wildtomato nearly 3-fold more LTR retroelements than cultivated one; and a higherC:G ratio. Most of the families of LTR retroelements identified previously in S. lycopersicum were also present in S. pennelli. Also, some familiesidentified as high copy number such as GypsySL_01 and CopiaSL_37 indomesticated tomato are also abundant in the wild species analyzed. However, weobserved differences in the LTR retroelements family time-insertion estimation,revealing recent divergences in retroelements populations between both species.The application of this information for molecular marker design will bediscussed. Funding: DTEC 0008/13 C-3 (AGENCIA, MINCyT,Argentina).