CENTRO DE INVESTIGACION Y DESARROLLO EN INMUNOLOGIA Y ENFERMEDADES INFECCIOSAS
Unidad Ejecutora - UE
congresos y reuniones científicas
VLPs pseudotyped with Variant-specific Surface Proteins of Giardia as an effective oral Influenza vaccine
RUPIL, LUCÍA; OMS, SERGIO; SERRADELL, MARIANELA DEL CARMEN; GARGANTINI PABLO RUBÉN; SAURA, ALICIA; LUJÁN, HUGO DANIEL
Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016
Sociedad Argentina de Investigación en Bioquímica y Biología Molecula
Oral vaccines possess attractive advantages over parenteral vaccines. However, there are certain drawbacks inherent to this form of immunization because the vaccinal antigen has to resist the passage through the upper gastrointestinal tract. Since Giardia Variant-specific Surface Proteins (VSPs) protect this parasite from the harsh environmental conditions of the intestine and have a remarkable intrinsic immunogenicity, we hypothesized that efficient oral vaccines can be generated by combining the benefits of Giardia VSPs as protective agents with the advantages of particulate immunogens like Virus-like Particles (VLPs). Here, we generated an Influenza A H5N1 VLPs pseudotyped with the extracellular domain of Giardia VSP1267 and orally immunized mice. When comparing to control VLPs without VSP1267, we found that the vaccines were able to induce the production of HA-specific antibodies in serum, as well as in mucosal fluids such as bronchoalveolar lavage and feces. When the different subtypes of IgG were analyzed in serum, it was found that both IgG1 as IgG2 showed a significant increase. In addition, a high number of IFNγ secreting HA-specific cells were present in spleen and mesenteric lymph nodes, indicating that both humoral and cellular immune response had been generated. Protection assays with live Influenza virus confirmed the efficiency of this novel oral vaccine, paving the way for the design of oral vaccines to other human pathogens.