INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Placenta?heart axis: abnormal cardiovascular development and VEGF expression after perigestational alcohol intake at mid-gestation in mouse.
Autor/es:
SOBARZO, C.; CEBRAL, E.; GUALDONI, GS; VENTUREIRA, MR; BARBEITO, C.
Lugar:
Ciudad Autónoma de Buenos Aires
Reunión:
Congreso; IFPA 2019 and VIII SLIMP; 2019
Institución organizadora:
SLIMP
Resumen:
The normal development of cardiovascular-placenta axis depends on VEGF and KDR expressions. Previously, perigestational alcohol consumption at mouse organogenesis produces altered VEGF expression in the embryo and labyrinth associated with vascular anomalies.Objectives: The aims were to assess the histopathology, ultrastructure and proliferation of the ventricular and atrial myocardium and endocardium of fetal heart, and morphogenesis of labyrinth (L) and junctional zone (JZ), and to evaluate the VEGF/KDR expression in the heart-placental axis at day 13 of gestation after cessation of perigestational alcohol intake at day 10 of gestation.Methods: Ethanol 10% in drinking water was administered to murine CF-1 females for 15 days before and up to day 10 of gestation (D10), and pregnancy continued with water until day 13 (treated females (TF). Control females (CF) consumed water. Morphological studies and VEGF/KDR expressions were performed in fetal hearts and placentas by histology (H-E and PAS), TEM, immunohistochemistry and western blot.Results: The cardiac wall thickness was reduced (p