INBIOMED   24026
INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Botryomycosis in NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice: A focal outbreak in an experimental colony
Autor/es:
ROJAS, PAOLA; GULIN, ERNESTO; PATACCINI, GABRIELA
Lugar:
Mar del Plata
Reunión:
Otro; Reunión Anual de Sociedades Biocientíficas.; 2019
Institución organizadora:
SAIC-SAFE-SAB-SAP-AACYTAL-NANOMEDar-HCS
Resumen:
NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ mice were purchased from Jackson Laboratory and maintained under conventional closed barriers in individually ventilated cages(Tecniplast®) with controlled temperature (20-22°) and relative humidity (50?70%) in a 12:12 h light: dark cycle. The animals were fed with autoclaved standard diet and carbon activated filtered water ad libitum. Cages were filled with sterile wooden chips and corn. The experimental protocols were approved by the IACUC. Mice (n=9; 6-12 months-old) included in patient-derived xenografts (PDX) studies exhibited solitary or multiple fibrose nodules of 0.50 cm, located near to the mouth and nose. The cases appeared randomly in cages for over five months. After clinical examination, differential diagnoses included bacterial orfungal infections, foreign body granuloma, sterile pyogranuloma or neoplasia. Mice exhibited poor body condition, weight loss and ruffed coat. Animals were submitted to necropsy. The nodules presented fibroelastic consistency at the cutting. Macroscopic description included hepatosplenomegaly, pale diffuse liver and uterus, ovaries and gastrointestinal tract inflammation, with coagulated blood content in stomach. At histological examination, there was a multifocal dermatitis, with diffuse bacteria colonies accompanied by fibrillar acidophilic material compatible with a Splendore-Hoeppli reaction, surrounded by neutrophils and pyocytes with moderate presence of epithelioid macrophages. The final diagnostic was botryomycosis, probably due to Staphylococcus spp. This is the first report of botryomycosis in NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice. Regarding their combined immunodeficiency condition, skin nodules should be early diagnosed, taking account botryomycosis as a differential diagnosis. Strict housing barriers and procedures have to be taken to the extreme in order to avoid or reduce the presentation and severity of this concomitant pathology.