Study of spermatogonia population in infertile patients with immune cells infiltrates

GONZÁLEZ, LUCAS NICOLÁS; GLIENKE, LEILANE; JACOBO, PATRICIA VERÓNICA; MÉNDEZ, CINTHIA SOLEDAD; FULCO, MARÍA FLORENCIA; THEAS, MARÍA SUSANA; AMARILLA, MARÍA SOFÍA; OXILIA, HERNÁN; SOBARZO, CRISTIAN

Mar del Plata

Congreso; SAIC.SAI.SAFIS 2018; 2018

SAIC, SAI, SAFIS

Azoospermia is linked to local testicular microenvironment deregulation,with inflammatory cells present in the 15% of testicular biopsiesof infertile patients. The aim of this study was to study the kinetics behaviorof spermatogonia (SP) population in infertile patients displayingtesticular immune cell infiltrates. Biopsies obtained from infertilepatients (Hospital. Clínicas, CABA) with obstructive and non-obstructiveazoospermia were classified according to morphologicalcriteria in: normal spermatogenesis (N, n=7), hypoespermatogenesis(Hypo, n=5) and severe hypospermatogenesis (SHypo, n=5).The number of immune cells was quantified by immunohistochemistryemploying the leukocyte common antigen marker (CD45) andthe number of SP (A dark, A pale and B) by morphological criteriain the same slides. The number of CD45 cells increased significantlyin Hypo and SHypo biopsies vs N (Mean±SEM N:22.150±2.510,Hypo:69.090±9.398, SHypo:95.180±12.54, p