UNDIFFERENTIATED SPERMATOGONIA CELL CYCLE ARREST CONTRIBUTES TO THE IMPAIRMENT OF SPERMATOGENESIS IN THE TESTIS UNDER A CHRONIC INFLAMMATORY PROCESS.

MENDEZ CINTHIA SOLEDAD; CRISTIAN SOBARZO; THEAS MARIA SUSANA; EUGENIA FERREIRO,; LIVIA LUSTIG; LUCAS GONZALEZ; PATRICIA JACOBO

obidos

Congreso; 20th European Testis Workshop (European Workshop on the Molecular and Cellular Endocrinology of the Testis),; 2019

Introduction: Infertility is a main worldwide problem that affects one of six couples at reproductive age around world being the male factor responsible of 60% for the consults for infertility. Inflammation involving the presence of interstitial cell infiltrates in the testis (orchitis) or in the testis and epididymis (orchi-epididymitis) generally associated with damage of seminiferous tubules (ST) occurs in approximately 15% of patients with male infertility. We developed and experimental model of autoimmune orquitis (EAO) useful to understand the mechanisms underlying spermatogenesis disruption. EAO is characterized by an interstitial lymphomonocyte cell infiltrate, moderate in focal phase and abundant in severe EAO, increased levels of nitric oxide (NO) and TNFα, apoptosis of post-meiotic germ cells (GC) and reduced proliferation of pre-meiotic GC (spermatogonia, EP, and preleptotene spermatocytes). We hypothesize that cell cycle of CD9+ undifferentiated EP is arrested and that NO and TNFα are involved in this event. Objective: Analyze the cell cycle of CD9+undifferentiated EP and evaluate if NO and TNFα are able to regulate cell cycle progression.