Soluble Guanylyl Cyclase Alpha1 Subunit is Involved in Cell Proliferation, Survival and Migration in Human Endometrial Tumor Cell Line ECC-1

PINO MT; RONCHETTI SA; DUVILANSKI BH; RICCI AG; CORDEIRO G; CABILLA JP

Buenos Aires

Congreso; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC). Reunión Conjunta de Sociedades de BioCiencias; 2017

Sociedad Argentina de Investigacion Clinica

Soluble guanylyl cyclase is an heterodimeric enzyme catalyzing cGMP formation composed by two subunits, alpha1 (a1) and beta1 (b1). Previously we have shown that a1 increased levels strongly correlate with E2-induced proliferation in E2-depentent tissues and a1 knock-down decreased cell proliferation in endometrial and breast cancer cell lines. The aim of the present study was to investigate the role of a1 in proliferation, survival and migration in the E2-responsive endometrial tumor cell line ECC-1.a1 expression was silenced through siRNA specific sequences using scramble sequences as control. Cells were incubated with or without 1 nM E2 for 48 h. Protein levels were measured by western blot. Apoptosis and mitosis were assessed by nuclear morphology (Hoechst). Cell cycle was studied by flow cytometry. Migration was determined through scratch motility assay. a1 knock-down (a1KD) significantly reduced cell proliferation markers´ expression both in presence and absence of E2 (PCNA as % of control (C), E2: 151.6±7.2*, a1KD:55±10*, a1KD+E2:93±11#; cyclin D1, E2:141.2±12.9*, a1KD: 33.3±18.2*, a1KD+E2: 40±15#; cyclin E, E2:127.6±11.6*, a1KD:29.8±14.2*, a1KD+E2:28±17#; *p